Johnny B and Johnny Smacks joined the radio station two years ago but will present their final drivetime show on May 31.
Head of RTE 2Fm, Dan Healy said: "After two very successful years, The 2 Johnnies are leaving 2FM. Johnny B and Johnny Smacks have brought their original South Tipp humour and interesting music choices to 2FM audiences right across Ireland. The listenership for the drivetime show has been very strong from the very start and the most recent listenership results continued that trend.
Johnny Smacks said: "We’ve had unreal craic over the last two and half years working on 2FM. We came in with the idea to have a laugh on the afternoons and cheer people up and get more mad scones on the radio from every county in Ireland telling stories and auld yarns. After two and a half years my jaws need a rest from all the laughing.
Johnny B added: "We’ve loved our time on the show. The team are legends, our sister Aifric O Connell, the great Aindriú de Paor, Producers Caroline, and Anita.Our listeners have been the funniest, maddest, soundest crowd alive. The stories they’ve come on air to tell have been outrageous. It’s made me proud to be Irish to talk with the nation every evening. The Parish Quiz has thrown up some wild characters, we’ve tried to reach every inch of the island of Ireland.
Dan Healy continued: "I want to acknowledge the great content created on the drivetime show by Aifric O'Connell, Aindriú De Paor and Emma Power who shared the airwaves with The 2 Johnnies over the period. Also, a very big thanks to the producers who worked on the show particularly Caroline Clarke and Anita Ward. We are fortunate to have such great talent working at 2FM and with them the station will continue to prosper".