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Discrimination between cyclic nucleotides in a cyclic nucleotide-gated ion channel - Nature Structural & Molecular Biology

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How the ancient messengers cAMP and cGMP deliver their messages WeillCornell NatureSMB

value of cGMP-CNBD was 3.3 ± 1.8 µM. The concentration of labeled His-C-linker-CNBD was kept constant , and the concentration of cNs was varied from 0.00305 µM to 100 µM.

Extended Data Fig. 2 Distributions of rupture forces of cAMP-CNBD or cGMP-CNBD bonds under various conditions.Force distribution of cAMP-CNBD unbinding following 0.02 s bond formation at varying pulling velocity .Force distribution of cAMP-CNBD unbinding following 1.00 s bond formation at varying pulling velocities .

 

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