Calu-3 were seeded in 300 µL into the apical chambers of 24-well transwell polycarbonate membrane inserts and 1.5 mL of medium was added to the basolateral chamber. Medium was changed daily from both the apical and basolateral chambers until cells formed a complete monolayer measured through maximal barrier resistance . On the day of the experiment, TEER of each transwell was measured to ensure that cell resistance levels were at a minimum value and roughly equivalent .
Immediately following ID injections, 150 µL of 10-kDa dextran conjugated to Alexa Fluor 680 was delivered intravenously . Two hours post-injections, mice were euthanized, and the dorsal dermises were removed and placed in petri dishes. Fluorescence signal accumulation in the mouse dermises was visualized using a fluorescence scanner at a wavelength of 700 nm. Leakage was quantified around the injection sites using Image Studio software .
physorg_com NatureComms Seems like covid propaganda continues. Science is so disgraceful nowdays... If the greatest genius who ever lived were alive today, they would be puzzled on what science has become.